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Then, 50 L of 0.2 mol/L acetic acid containing d9-TMAO was added, followed by 150 L ice-cold trichloroacetic acid, and samples were incubated at 4 C for 10 min to precipitate proteins

Al-Gadani, Y., El-Ansary, A., Attas, O., & Al-Ayadhi, L

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A next generation multiscale view of inborn errors of metabolism