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Analytical Documentation Context Includes analytical review and quality documentation

Future studies using varied diets are needed to determine whether the predominance of Ileibacterium in the cecal microbiome holds pathological relevance

[Online] doi: 10.1093/ajcn/77.2.406

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Our observation that intracellular NA concentrations did not increase following NNMT inhibition extends previous studies that showed NA concentrations were largely unaffected following NNMT knockdown with either adenovirus[39] or anti-sense oligonucleotides (ASOs).[12] It is possible that significant accumulation of NA in adipocytes (and other tissues) is prevented by rapid conversion of NA to NMN and subsequent NAD + synthesis or transport to the extracellular environment.[27] Importantly, NNMT inhibition potentiated the absolute intracellular concentrations of NAD + and SAM, without altering the levels of NA and SAH in the adipocytes