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Hydrogen deuterium exchange mass spectrometry (HDX-MS) HDX experiments were performed with or without liposomes (final concentration 0.1 mg/mL) containing brain porcine phosphatidylethanolamine, brain porcine phosphatidylserine and brain porcine phosphatidylinositol 4,5-bisphosphate in a 10:5:1 molar ratio (Avanti Polar Lipids) in a total reaction volume of 50 l with a final concentration of 400 nM PLC, 1mM NaF, 30M AlCl 3 and 600 nM G q or G where appropriate

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Agrawal M, Christensen HS, Bgsted M, Colombel JF, Jess T, Allin KH

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