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Description
The GST construct (Amersham Biosciences) in pGEX-4T-3 vector was purified by manual glutathione-affinity chromatography using Glutathione Sepharose 4 fast flow beads (#17-5132-01, GE Healthcare) and eluted with 10 mM reduced glutathione (#11514, SRL), 150 mM NaCl and 2 mM DTT
Pollicino et al., 2023)
Early detection saves lives.
[7] Commonly Reported Effects The peptides most frequently observed effects are mild and transient
We observed by optical microscopy that a GUV with an initially smooth spherical shape (Fig
186 Other Hormones: Luteinizing hormone (LH) and follicular stimulating hormone (FSH)
