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It should be pointed out that Griffith [11] originally validated this method in plasma where the artifactual oxidation of GSH is minimal, i.e., tolerable, because the GSH/GSSG ratio in plasma is much lower than in cells (~2 instead of ~400) [3], but this procedure has later been applied broadly to whole blood, red cells or other tissues in which GSH massively exceeds GSSG without proper modification

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Previously, inhibition of ferritinophagy by targeting the NCOA4-FTH1 interaction was shown to reduce the LIP, thereby inhibiting ferroptosis and promoting cell survival [131]

doi:10.1016/j.mrfmmm.2005.02.023 Follow this link Frykberg RG, Andersen C, Chadwick P, et al