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Rapid disruption of intestinal barrier function by gliadin involves altered expression of apical junctional proteins

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Step 3: Add diluent slowly Use a syringe or calibrated pipette to add diluent slowly down the inner wall of the vial rather than directly onto the powder

Serum limitation-induced senescent fibroblasts model and effect of FOXO4-DRI Passage 3 fibroblasts derived from keloids and normal skins were cultured in a Dulbeccos Modified Eagles medium (DMEM, Hyclone, Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) to 90% confluence, and then cultured in a DMEM containing 2% FBS for 3 days to construct a serum limitation-induced senescent fibroblast model