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All CD1 mice used in defeat experiments were pre-screened according to two criteria: across three 180-second screening sessions conducted once daily, each CD1 mouse was required to initiate attacks in at least two consecutive sessions, and the latency to initial aggression, recorded during each session, had to be less than 60 s

A 1988 study flagged zinc as a 5-alpha-reductase inhibitor in vitro

For instance, Ishikawa cells are prone to losing cellular phenotype (such as loss of hormone receptor expression), exhibiting genetic modifications during prolonged culture, and showing instability at microsatellite loci