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Nuclear extracts from the v-Src-transformed mouse fibroblasts (NIH3T3/v-Src) containing activated STAT3:STAT3 dimers [30, 31] were first incubated with increasing concentration of R001 for 30 min at room temperature prior to incubating with the radiolabeled high-affinity sis -inducible element (hSIE) probe that binds STAT3 and performing EMSA analysis in vitro [10, 11, 25, 32]

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