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Transformation of yeast cells for ABP1-mTurquoise tagging Previously generated competent cells were thawed quickly and spun down at 13000 x g for 2 min at 4 C, discarding the supernatant

A Glutathione normally exists in the reduced form (GSH) in the body but is converted to the oxidized form (GSSG) by stimuli such as oxidative stress

Concurrently, the gut microbiota, comprising diverse microorganisms, significantly influences host metabolism and immune responses

If this process is inhibited, for example by thiamine deficiency, lactate will build up

Neuron 82 , 11011114 (2014)

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