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This muscular activity, which is very different from a real contraction, acts like a pump and is decisive in provoking a sharp increase in blood flow to the stimulated muscle
Preparing your glutathione beads Loading your GST-fusion protein onto the glutathione beads Adding the interacting proteins Eluting the GST-fusion and interacting proteins Materials needed for GST pull-down assays Preparing glutathione agarose beads Loading GST-fusion proteins onto glutathione beads Adding interacting proteins Eluting the beads Analyzing pull-down results What is a GST pull-down assay
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