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GI events (nausea, vomiting, diarrhea) were the dominant safety signal and occurred at rates higher than reported for Phase 2 retatrutide and higher than for comparable dual or mono-agonist trials

MTT assay The viability rate of cells on the PU-CA scaffold was measured using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

Trypan blue was used to visually inspect and count the number of nuclei

doi: 10.3233/jpd-2012-11052

Results vary, but many patients notice reduced shedding within a few weeks and visible thickening within 36 months

Moreover, a significantly greater proportion of patients with baseline prediabetes reverted to normoglycemia in the semaglutide vs