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doi: 10.3109/10717544.2013.838713 [DOI] [PubMed] [Google Scholar] Morello Maria et al

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Indeed, when GSAAT is reduced with gradually increasing amounts of DTT prior to incubation with NEM, either one Cys (Band 1, with 0.1 mM DTT) or no Cys residue of GSAAT is accessible for mPEG binding (with DTT 1 mM) ( Figure 2C )