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Briefly, lentiviral particles were produced in 293T cells by co-transfection of psPax2 packaging vector (Addgene), VSVG envelope plasmid (Addgene), pDBR (mock), or pDBR-IL-10 plasmid using Lipofectamine 2000 (Invitrogen)

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165 ROS also interact with calcium signaling at the mitochondrialER interface, where Ca 2+ surges induce mitochondrial swelling and rupture via disrupted ion exchange and adenine nucleotide translocase (ANT) channel dysfunction