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Peroxynitrite Although the idea that oxidant stress is critical for APAP-induced cell death was largely accepted by the late 1990s, the mechanisms of ROS-induced cell death remained unclear

The protein sample (250 g protein /30 L) was mixed with 270 L of UA (100 mM TrisHCl (pH 8.5) containing 8 M Urea) in the filter unit, and centrifuged at 14,000 g for 15 min

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2021;5:nzabO69

Acknowledgements This work was supported by grants from the Ministerio de Ciencia, Innovacin y Universidades (MCIU) Plan Estatal de I+D+I 2018, a la Agencia Estatal de Investigacin (AEI) y al Fondo Europeo de Desarrollo Regional (MCIU/AEI/FEDER, UE): RTI2018-097455-B-I00

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