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Glutathione is a tripeptide amino acid produced in the liver primarily from cysteine.* It acts as a cellular antioxidant by inhibiting free radical formation.* Glutathione has been called the master antioxidant, because it recycles the antioxidants vitamins C and E and also helps to regulate immune cells and detoxify the body.*

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Relative to other affinity interactions, the binding between GST and glutathione is pretty slow, so youll want to incubate your protein of interest with the beads for around 30 minutes to an hour before uncapping the column and letting the flow-through drip out the bottom