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Histol Histopathol 1991;6:21-24

6 (2023): 11621176, 10.1002/jor.25470

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The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 m 2 cm, nano Viper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 m inner diameter, 3 m resin) in buffer A (0.1%formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology

Shubhrajit Mantry and Sanjeeb Kumar Kar would like to acknowledge Sarala Birla University, India for providing the facilities to draft this manuscript, and encouraging for conducting various research activities