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Reagent 3 w orking solution preparation : Add 200mL(100T-48S)/100mL(50T-24S) double distilled water to dissolve Reagent 3 , Stored in plastic bottle (given by our Institute) for 6 months

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5 , e1492 (2015)

In eukaryotes and aerobic prokaryotes, GSTs are grouped into at least four major protein families: cytosolic GSTs, mitochondrial GSTs, microsomal GSTs, and bacterial Fosfomycin-resistance proteins [40, 42, 58-59]

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In addition studies have also shown that the polysaccharide isolated from the Tulsi could prevent oxidative damage to liposomal lipids and plasmid DNA induced by various oxidants such as iron, 2,2-azobis (2-amidino-propane) dihydrochloride and gamma radiation, besides scavenging important reactive oxygen species like superoxide radical and hydrogen peroxide and to also inhibiting xanthine oxidase.[] The flavanoids orientin and vicenin is reported to possess good free radical-inhibiting activity in vitro , and the effect was better than that caused by dimethyl sulfoxide.[] Both compounds inhibited free radical formation in the absence of ethylenediaminetetraacetic acid and did not possess pro-oxidant activity.[] In addition, studies with Escherichia coli mutants' deficient in superoxide dismutase (SOD) and catalase genes have also shown that both orientin and vicenin were equally effective in reducing radiation and the chemical oxidant-induced cytotoxicity and to mediate the effects through free radical scavenging activity.[] Simultaneously animal studies have also shown that administering Tulsi extract increases the basal levels of free glutathione (GSH) and the antioxidant enzymes glutathione transferase (GST), reductase, peroxidase, and SOD and to concomitantly decrease the levels of lipid peroxidation in both sham and irradiated mice.[] Studies have shown that both the flavonoids prevented radiation-induced lipid peroxidation in the liver of mice whole body exposed to 3 Gy of gamma radiation.[] Pretreatment with Tulsi (40 mg/kg body weight for 15 days) reduced the levels of lipid peroxidation and prevented the depletion of GSH in both kidneys and salivary glands induced by high-dose of 131 I (3.7 MBq).[] Oral administration of the hydroalcoholic extract of Tulsi (200 mg/kg b