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Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 L of RNase A (10 mg/mL in TrisEDTA buffer, pH 8.0) solution were added

In this regard, elevated intracellular calcium levels have been detected in cataractous lenses, and more recently, alterations in Ca 2+ signaling have been determined in human lens epithelial cells during cataract progression [39]

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Different ones had different caffeine levels, but the chlorogenic acid levels varied by more than 30-fold