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Additionally, it can be caused by either a lack of B12 in the diet, gastritis, pernicious anemia, digestive diseases (e.g., Crohn's disease and celiac disease), surgery on the GI tract, alcohol use disorder, transcobalamin II deficiency, or Sjgren's syndrome [9]

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The resulting solution is still colorless and contains no particles observable to the naked eye

It fails in the sense that the agitation can denature the peptide

For correct solution concentration and portion amounts after adding solvent you can use our peptide calculator

Comparing the Essentials: A Research Perspective Benefits of Using Bacteriostatic Water for Peptides in Research Why is bacteriostatic water for peptides the gold standard in the lab