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[] According to the latest estimates of the global burden of liver cancer in 2020, liver cancer cases and deaths are projected to increase by more than 55% by 2040

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The thermocycling conditions consisted of a denaturation at 95C for 20 sec, followed by 45 cycles of denaturation at 95C for 15 sec, annealing at 60C for 15 sec and extension at 72C for 20 sec

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In addition, signs of anemia to look out for in cats include pale gums, increased heart rate, increased breathing rate, lethargy and weakness (sleepiness), decreased appetite, increased water intake, and collapse