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The reduced form is considered particularly active

clinical note

6C and I) in GLAG-66 cells treated with APS and DB

To purify C0362 proteins, the cell pellet was resuspended in ice-cold Buffer A (50 mM Tris, 300 mM NaCl, 0.5 mg/mL lysozyme, 2 mM dithiothreitol (DTT), 20 mM imidazole, pH 8.0) and lysed by two passages through a microfluidizer at ~25,000PSI

We also speculate that the identification of these deregulated sperm proteins related to low intratesticular T may represent, if validated by further studies, putative non-invasive indirect biomarkers of intratesticular T

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