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Elevated TMAO levels have been linked to an increased risk of cardiovascular diseases, indicating a complex relationship between carnitine intake and health outcomes [14,15] (Figure 1)

Transformed bacteria were cultured in LB medium at 37 C until the optical density (OD600) reached 0.8

Appl Environ Microbiol 68:45174522 Mesiano AJ, Beckman EJ, Russell AJ (2000) Biocatalytic synthesis of fluorinated polyesters

9 percent, respectively)