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Cells were washed with PBS 3 times then fixed with 4% PFA for 30 minutes

Investigating the Origin of the Plantaris Muscle invivo Using Ultrasound

Features/Benefits: Energy Boost: Promotes energy production and may help fatigue

doi: 10.1111/j.1528-1167.2008.01870.x 82 NethB

A more in-depth analysis of the effect of oxygen levels permitted conditions to be optimized [63, 64] and showed that -butyrobetaine production was dramatically decreased when the biotransformation was performed under aerobic conditions as a consequence of the inhibition of crotonobetaine reductase by oxygen [14, 16, 63, 65]

(PubMed) Schmelzer C, Kubo H, Mori M, et al