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[DOI] [PMC free article] [PubMed] [Google Scholar] Bjrklund G, Aaseth J, Ajsuvakova OP, Nikonorov AA, Skalny AV, Skalnaya MG, Tinkov AA

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Manual ELISAs generally show lower precision than automated platforms, with intra-assay coefficients of variation (CV) of 5.4-6.03% and inter-assay CVs of 5.6-6.7%, whereas automated assays achieve lower CVs (1-2.6% and 2.5-3.9%, respectively), higher sensitivity (detection limits as low as 0.0098 ng/mL), and greater throughput 70,71,72