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Supernatant and lysed mitochondria were separated by centrifugation at 20,000 g for 45 min

Several sleep deprivation approaches have been developed for rodents (Havekes et al., Chronically restricted sleep is an adverse environmental factor that affects a vast number of people, a consequence of living in an age of increasing access to technology, societal pressures and the expectation of long workdays

J Cel Physiol 182:163170 Spector D, Labarre J, Toledano MB (2001) A genetic investigation of the essential role of glutathione mutations in the proline biosynthesis pathway are the only suppressors of glutathione auxotrophy in yeast

doi: 10.1002/aur.1934
