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Pathological oxysterols can displace or promote an increase in cholesterol oxidization, leading to reduced GABA A receptor surface stability and faster receptor internalization reducing inhibition (Sooksawate and Simmonds, 1998

doi: 10.1007/s00018-009-0145-9

Dela Cruz MT, Patel S, Ngo L, Sullivan K

Absorbance at 490 nm was measured using a Synergy H1 BioTek (Agilent) spectrophotometer after a 3-hour dark incubation of cells with the reagent

These uremic toxins can have toxic effects on skeletal muscle cells by inhibiting cell proliferation and viability (Figure 2)