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SIRT1 or SIRT3 was co-immunoprecipitated and eluted with elution buffer (Pierce # 21004), and their activities were measured using the SIRT GLOTM assay kits (Promega # G6450), according to the manufacturers instructions
In these studies, the amount of 5-amino-1MQ administered was 10-20 mg/kg per day, allowing for precise measurement of its effect
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As this form of B12 is not a licensed Prescription Only Medicine (POM), this supplement can be purchased online without prescription
