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A blood sample was collected from all subjects before and after the peptide presentation

FuGENE 6 Transfection Reagent (Promega) was used to transfect AC16 cells, which were seeded in 96-well plates (50,000 cells), with human PPARGC1A promoter containing pGL3-BV plasmids according to manufacturers protocols

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Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice