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Nuclear NAD + is generated by nuclear NMNAT1 and is largely utilized by PARPs for DNA damage response and nuclear sirtuins such as SIRT1, SIRT6 or SIRT7 for epigenetic deacetylation 82,86

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Baseline measurements should confirm AMPK suppression (reduced phosphorylation status in muscle or liver) and NAD+ depletion (low tissue NAD+ levels or elevated NNMT enzymatic activity)