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This mixture was centrifuged at 15,000 r/min for 10 min at room temperature

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Protocol: 1.Set spectrophotometer to the right wavelength

Remaining chemistry parameters alanine aminotransferase (ALT), aspartate transaminase (AST), total cholesterol (Chol), creatinine (CREA), glucose triglyceride (TG), blood urea nitrogen (BUN) and LDL cholesterol were analysed using the Pentra C400 (Horiba Medical)

The ongoing phased replacement of older, more toxic NRTIs (e.g., d4T, AZT) with newer agents such as tenofovir alafenamide (TAF) and integrase strand transfer inhibitors (INSTIs), which have minimal impact on mtDNA, is a critical step in mitigating iatrogenic mitochondrial harm (164166)

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