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A team of scientists at the Wyss Institute for Biologically Inspired Engineering at Harvard University has discovered why long-term treatment with many common antibiotics can cause harmful side effectsand they have uncovered two easy strategies that could help prevent these dangerous responses.

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Purification of GST-tagged fusion proteins Glutathione affinity purification, like many other types of affinity purifications, involves three key steps: 1) binding the target protein to the beads, 2) washing away contaminating proteins, and 3) eluting your protein of interest (Figure 3)