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Bacteriostatic water is sterile water containing a preservative commonly used in laboratory and analytical environments

The mapping of filtered reads, as well as the calculation of methylation percentages at CG loci, were performed using the Bismark software 22

Specifically, we analyzed affected brain areas (04), cerebral (NTP-7, Level 3), cerebellar cortex (NTP-7, Level 6), and the hippocampus, thalamus, and hypothalamus (NTP-7, Level 3) as follows (score 0 indicates no histopathologic change): score 1: small, patchy, complete or incomplete infarcts (10% of area affected)