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The threshold for squeezing unmethylated DNA through 1.8 nm pore is about 3.6 V while fully-methylated DNA shows a threshold 2.7 V

Methylation levels of SFRP1 , SFRP2 , SDC2 , and PRIMA1 were determined using a MethyLight assay after cfDNA isolation and bisulfite conversion of samples ( SOX21 gene promoter methylation, serving as a fecal-based noninvasive biomarker for early diagnosis of colorectal cancer (Moradi et al., 2021)

Nevertheless, the trial-specific modified device demonstrated insufficient reliability in a clinical study, necessitating its mid-trial replacement with the Precision Olfactory Delivery (POD) device (Impel Pharmaceuticals, Seattle, WA) [67]

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