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Moreover, the use of self-cleaving tags can simplify the entire process at reduced costs, considering that the use of additional steps is not required for the separation of the target protein from the protease and the affinity tag (Wang et al., 2010)

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In Long COVID, this endothelial damage promotes immunothrombosis, the formation of microscopic blood clots (microclots) that block tiny capillaries and prevent oxygen from reaching vital tissues like the brain and muscles

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NF-B is a transcription factor family activated by pro-inflammatory cytokines including TNF and IL-1 B with a critical role in regulating both innate and adaptive immune responses, lymphocyte differentiation and proliferation, and cellular stress responses [32]

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