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doi: 10.1038/s41598-021-86425-6 193 ProalAMarshallT

Tailoring materials for modulation of macrophage fate

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After 4 days, the TS2/16 scFv-Fc was captured from the conditioned media with CaptivA HF protein-A affinity resin (Repligen, CA-PRI-0100) and eluted with IgG elution buffer (Thermo Scientific) containing 300 mM NaCl and neutralized with 100 mM Tris pH 8.0 to reach eventually pH 7.0

PMC 1448331

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