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They were then incubated with oxygenated isolation buffer [5 mM Na 2 EDTA, 10 mM HEPES (pH 7.4), 0.5 mM DTT, 0.25% BSA, 2.5 mM D-glucose, 2.5 mM L-glutamine, and 0.5 mM dl--hydroxybutyrate sodium salt, oxygenated with an O 2 /CO 2 mixture (19:1, v/v)] at 37 C for 40 min to isolate the upper villus epithelial cells
The results showed that the cells expressing GST-EGFP-GPI DAF , GST-EGFP-GPI BY55, and GST-EGFP-GPI CEAM7 exhibited comparable proliferation ability to cells expressing the single EGFP only (Supplementary Figure S1)
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