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For immunofluorescence staining, brain tissues were dissected out, post-fixed by 4% paraformaldehyde (PFA) for 12 h, embedded in paraffin, cut into 5 mm sections and mounted on slides for subsequent staining

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Vitamin B12 injections are often used as a treatment for people with vitamin B12 deficiency, a condition that can lead to a range of health problems, including fatigue, weakness, numbness and tingling in the extremities, and difficulty concentrating

Addressing underlying causes is essential: Dietary modification and supplementation for vegans/vegetarians Management of gastrointestinal disorders affecting absorption Screening for coeliac disease if indicated Review of medications that may contribute to deficiencypatients should not stop prescribed medications such as metformin or proton pump inhibitors without consulting their doctor For patients on long-term metformin, the MHRA recommends considering vitamin B12 testing in those with symptoms or risk factors for deficiency, with periodic monitoring in at-risk groups

It has been declared, that neurotoxic 6-hydroxydopamine (and also superoxide radical) is able to release free iron from ferritin which in turn may participate in Fenton chemistry, forming ROS and initiating lipid peroxidation (Monteiro and Winterbourn 1989)

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