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Hypoxia reduces the inhibitory effect of IL-1beta on chondrogenic differentiation of FCS-Free expanded MSC

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The resin was washed with 10 resin volumes of the washing buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 20 mM Imidazole) and eluted with the elution buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 400 mM Imidazole)

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