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36, e5362, 53

doi:10.1038/s41394-018-0100-9 19

Dendritic Cells The PLGA microparticles induce tolerogenic dendritic cells (tDCs), promoting regulatory T cell (Treg) expansion and reducing arthritis symptoms

Furthermore, many GDPs such as 3,4-dideoxyglucosone-3-ene (3,4-DGE) are reactive carbonyl compounds which along with glucose form advanced glycation end-products (AGEs), binds to free amino groups on membrane proteins or lipids contributing to pathophysiological alterations in the peritoneal membrane (Linden et al., 2002

In previously published protocols different types of derivatization reagents (CMQT or CMLT), different pH of the buffer used in the protocols (7.4) and shorter time of sample reduction and derivatization (3 min) were applied (Tables 3 and 4.) Changing the conditions of reduction and derivatization increases the efficiency of this process, which may give higher final concentrations of the analytes