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Histology (H&E), IHC (MUC2 and Nrf2), ROS (ELISA), gene expression (qRT-PCR), and protein levels (Western blotting) were analyzed in colon tissues

John Clarke 1,4 , PhD

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Biochemical assessments measured oxidative stress markers [superoxide dismutase (SOD), catalase, and glutathione (GSH)], proinflammatory cytokines (TNF, IL-6, and IL-1), and brain monoamines [dopamine (DA) and acetylcholine (ACh)]

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