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Elife 3, e03421

Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells

doi: 10.1096/fba.2021-00022

Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

In hepatocellular carcinoma triggered by MYC and KEAP1 inactivation, fructosamine-3-kinase (FN3K), a kinase that triggers protein de-glycation, mediates NRF2 de-glycation that stabilizes NRF2 and executes its oncogenic function [28]

Furthermore, the prevention of hydroperoxide-induced ferroptosis requires the utilization of selenium by GPX4 [34]