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Migrated cells were imaged with a microscope and quantified with the ImageJ 2.0.0 (Fiji) software
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Furthermore, the treatment with NMN resulted in a significant upregulation of adipose triglyceride lipase (ATGL) expression in subcutaneous fat, which is in line with the observed alterations in fat mass and adipocyte size ( + may exert beneficial effects on lipid profiles by modulating the expression of lipid-metabolizing enzymes

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