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Yes, storage protocols differ significantly

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We performed the reactions for a determined number of cycles (in two consecutive cycles (2223 for GAPDH and 2728 for eNOS ) in the logarithmic phase of PCR reaction, cDNA was amplified and analyzed)

Kisspeptin/GnRH1 System in Leydig Cells of Horse (Equus Caballus): Presence and Function

This might sound like a minor biochemical reaction