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Description
Tau protein squired by molecular chaperones during Alzheimers disease
Methods Cell culture NCI-H1299 wild type (WT), NCI-H1299 HIF-1 knockout (KO) and HIF-2 KO cells were cultured in RPMI media supplemented with 10% fetal bovine serum (FBS) and maintained at 37 and 5% CO 2
Our systematic protocol evaluates the impact of the helper molecules, the pH, buffer system and time on the protein refolding process in a high-throughput fashion
Nat Biotechnol 24(1):4647
Maddux MS, Barriere SL
& Naghashian, F
