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J Biomed Res

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

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Longer trials are needed for researchers targeting alterations in body composition or other GH-associated advantages in their subjects