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Aliquots of protein lysates were electrophoresed on a sodium dodecyl sulfatepolyacrylamide gel, transferred from the gel onto a PVDF membrane, and blocked with 5% BSA for 1 h

Jauniaux E, Cindrova-Davies T, Johns J, Dunster C, Hempstock J, Kelly FJ, Burton GJ: Distribution and transfer pathways of antioxidant molecules inside the first trimester human gestational sac

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Increased NADPH oxidase-derived superoxide is involved in the neuronal cell death induced by hypoxia-ischemia in neonatal hippocampal slice cultures

Another deleterious pathology associated with mTOR activation is insulin resistance which can lead to suppressed glucose metabolism and insufficient ATP production (Di Domenico et al