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As both H 2 O 2 and BCNU exposure led to excessive cellular GSSG accumulation, their impact on intracellular protein-GSSG adduct formation (protein S -glutathionylation) was subsequently examined in HepG2 cells by western blot analysis using a monoclonal GSSG antibody that specifically binds to GSSG-modified proteins

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Beyond single-gene modulation, ischemic stroke induces broad transcriptomic shifts affecting inflammatory networks, synaptic signaling pathways, mitochondrial function, and extracellular matrix remodeling

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