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Description
overhangs removed by Phusion DNA polymerase 120 ) carrying a sgRNA targeting KGA stop codon (5ATCTTGATGGATTGTTGTAA3) were co-transfected with a pUC19 with constructed donor sequence (1 kb 5 homology arm, gly-ser-thr linker, mKO2, gly-ser rich linker, 2 A self-cleaving peptide from porcine teschovirus-1, zeocin resistance, stop codon, and 1 kb 3homology arm) into HEK293 cells
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The first step is routine screening for sleep and circadian disturbances in all ADHD patients
