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Microinjection was performed using the following injection mix: KCl (25 mmol L 1 ), Hepes pH 7.4 (7.5 mmol L 1 ), tracrRNA (200 ng L 1 ), dpy-10 crRNA (150 ng L 1 ), dpy-10 ssODN (13.75 ng L 1 ), adh-5 sgRNA (300 ng L 1 ), adh-5 ssODN (100 ng L 1 ), Cas9 (416 ng L 1 , NEB, USA) 59

Biochimie 116:5260

To be fair, oral contraceptive use isnt the only reason for low magnesium levels since experts estimate that more than 50% of the US population is deficient

Dequan Xus lab, Huazhong Agricultural University) and pEGFP-C1 and pmCherry-C1 vectors (gifted from Prof

(45) Bastin, J.-F