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Briefly, cotton stoppered shake-flasks were used with a working mass of 50 g containing water insoluble solids (WIS) content of 5 or 10% (w/w) steam-pretreated spruce (SEKAB E-Technology rnskldsvik, Sweden)

Karmakar, S.K

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As the observed effects could be due to increased systemic glutamine availability, we treated HFD-fed mice with intraperitoneal glutamine injections for 2 weeks and measured the expression of genes encoding factors involved in browing and bioenergetic pathways in iWAT

Three to five images/sample were analyzed, with automated analysis confirmed by visual inspection